Tris Base Powder CAS 77-86-1

 , also known as {{0}}amino-2-hydroxy-1,3-propanediol or Tris(hydroxymethyl)aminomethane, is an organic compound with important biological functions. The chemical structural formula is HOCH ₂ (CH ₂ OH) ₂ CH ₂ NH ₂, which is a ternary alcohol amine with one alcohol hydroxyl group and two primary alcohol hydroxyl groups. Its molecular formula is C3H8NO3, CAS 77-86-1, with a molecular weight of 121.1. It is in the form of a white crystalline powder, odorless, and non hygroscopic. It is stable in dry environments, but may absorb moisture and cause deliquescence in humid environments. Soluble in water and can dissociate into hydrogen ions and tris ions under acidic conditions. Its aqueous solution is slightly acidic, with a pH value of approximately 7.0. In addition, it is also soluble in certain organic solvents, such as ethanol and glycerol. Stable at room temperature, but may undergo decomposition under high temperature and strong acid conditions. It is sensitive to light and air, and may be oxidized when exposed to air. As an important organic compound, it possesses various physical properties that have broad application value in fields such as biology, medicine, and materials science.

Addition information of chemical compound: Melting point 167–172 degree ( lit. ), Boiling point 219 - 220 degree 10 mm Hg ( lit. ), Density 1,353 g/cm3, Vapor pressure 0.0267 hPa ( 20 degree ), Refractive index 1.4170 (estimate), flash point 219 – 220 degree / 10 mm, Storage conditions 20-25 degree , Solubility H2O : 4M at 20 degree

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1. Buffer and solvent: As a common buffer, it is widely used in research in the fields of biology, medicine, and chemistry. It has a high buffering capacity, can stabilize the pH value in the solution, and maintain electrical neutrality over a wide pH range. In addition, it can also be used as a solvent to dissolve various compounds, including biomolecules and other organic or inorganic compounds.
2. Protein purification: commonly used in the purification process of proteins. It combines with proteins to form precipitates, which can effectively remove impurities from the solution after centrifugation or filtration. This purification method is commonly known as "salt precipitation method" and is a simple and effective protein purification method.

3. Electrophoretic buffer: Often used to prepare electrophoresis buffer, especially in the preparation of TAE or TBE buffer for DNA and protein electrophoresis. These buffers can stabilize the current and maintain pH stability during the electrophoresis process.
4. Biochemical research: widely used in biochemical research. It can act as an activator or inhibitor of enzymes and participate in various biochemical reactions. In addition, it can also be used to prepare cell culture media and cell separation solutions.
5. Clinical diagnostic reagents: Used as reagents in clinical diagnosis, for preparing diagnostic kits and calibration standards, etc. It is typically used in conjunction with other compounds to detect and analyze specific substances in biological samples.
6. Analytical Chemistry: Commonly used in ion chromatography and mass spectrometry in analytical chemistry. It can serve as an ion pair reagent to improve the separation efficiency of ions and the accuracy of analysis. In addition, Tris(hydroxymethyl)aminomethane can also be used as a stationary phase for gas chromatography, improving separation efficiency and column performance.
7. Laboratory chemicals: As a type of laboratory chemical, it is widely used in laboratory research and experiments. It can participate in chemical reactions as a reactant or be used as a solvent to dissolve other compounds. In addition, it can also be used to prepare standard curves and control the pH value of samples.
8. Other applications: In addition to the common applications mentioned above, Tris base also has other applications. For example, it can be used as one of the components in flux and cleaning agents, as well as an intermediate in the preparation of other chemicals and materials.

1. Add 100 mL of ammonia to a round bottomed flask and stir with a glass stirrer.

2. Slowly add trichloroacetaldehyde (10 g, 0.073 mol) dropwise into ammonia solution, control the dropwise acceleration to ensure that the reaction temperature does not exceed 35 degree .

3. Continue stirring the reaction solution at room temperature for 30 minutes, then add an appropriate amount of sodium hydroxide solution (10 g, 0.35 mol) to achieve a pH value of 8-9.

4. Transfer the reaction solution to a rotary evaporator, use methanol or ethanol as a solvent for rotary evaporation, remove ammonia and water, and obtain the crude product of Tris base.

5. Dissolve the crude product in hot water and then perform recrystallization. Filter and dry the crystals to obtain pure Tris base.

₃ (aq) + CHCl₃₄Cl (aq) + CH₂

₂O (aq) + H₂₂

CH2OHCHOHCHO (aq) + NH₄₄Cl (aq) + H₂O (l) + Tris base (aq)

The synthesis of Tris base through the reaction of N-bromosuccinimide (NBS) with 2-amino-1,3-propanediol is a commonly used method. The following are the detailed steps and chemical reaction equations of this method:

1. Synthesis of NBS:

1.1 Add an appropriate amount of methanol or ethanol to a round bottomed flask and stir with a magnetic stirrer.

1.2 Slowly add succinimide (10 g, 0.073 mol) dropwise to bromine (10 g, 0.073 mol), control the dropwise acceleration to ensure that the reaction temperature does not exceed 35 degree .

1.4 Recrystallize crude NBS with methanol or ethanol, filter and dry to obtain pure NBS.

2. Synthesis of Tris base:

2.1 Mix 2-amino-1,3-propanediol (10 g, 0.073 mol) with an appropriate amount of sodium hydroxide solution (10 g, 0.35 mol) and stir with a magnetic stirrer.

2.2 Slowly add NBS (10 g, 0.0365 mol) dropwise to the above solution, control the droplet acceleration to ensure that the reaction temperature does not exceed 35 degree .

2.3 Continue stirring the reaction solution at room temperature for a certain period of time until NBS fully reacts to obtain the crude product of Tris base.

2.4 Recrystallize the crude Tris(hydroxymethyl)aminomethane with methanol or ethanol, filter and dry to obtain the pure Tris base.

₄H₄₂ + C₃H₉₂₂Zn + NaBr + CO₂ + H₂O

The NBS used in this method has high activity and can be substituted with 2-amino-1,3-propanediol to obtain the target product

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